Journal: Frontiers in Immunology

Article Title: Humoral Immune Response Diversity to Different COVID-19 Vaccines: Implications for the “Green Pass” Policy

doi: 10.3389/fimmu.2022.833085

Figure Lengend Snippet: Spearman’s correlation analysis of serum anti-receptor-binding domain (anti-RBD) antibody titers and neutralizing activity in 70 participants in the study (22 vaccinated with BNT162b2, 9 with mRNA-1273, 27 with ChAdOx1 nCov19, 9 with Ad26.COV2.S, 1 COVID-19 convalescent, and 2 with mixed vaccines). (A) Correlation plot of anti-RBD antibody titers versus neutralizing activity (percentage inhibition of RBD-ACE2 binding) assessed through the cPass™ ELISA-based assay. (B) Correlation plot of anti-RBD antibody titers versus neutralizing activity assessed through IgG/Neutralizing Antibody Rapid Test. (C) Correlation plot of neutralizing activity evaluated through cPass™ ELISA-based assay and IgG/Neutralizing Antibody Rapid Test cassettes. Trendlines, Spearman’s r , and p -values are also represented (statistical significance for p < 0.05).

Article Snippet: Some of them have high costs, require trained personnel, and can only be carried out in a Biosafety Safety Level 3-equipped laboratory, whereas others, such as the cPass surrogate virus neutralization test (sVNT) (GenScript, Piscataway, NJ, USA) used in this work, are ELISA-based assays and only require optical density readers.

Techniques: Binding Assay, Activity Assay, Vaccines, Inhibition, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Humoral Immune Response Diversity to Different COVID-19 Vaccines: Implications for the “Green Pass” Policy

doi: 10.3389/fimmu.2022.833085

Figure Lengend Snippet: Neutralizing activity evaluated by cPass™ ELISA-based SARS-CoV-2 Neutralization Antibody Detection Kit in 70 sera from differently vaccinated individuals. Serum samples were considered positive when ≥30% inhibition was measured, as shown by the red line in the graph. (A) Percentage inhibition of receptor-binding domain–angiotensin-converting enzyme 2 (RBD-ACE2) binding within different vaccination groups (see also Table 3 ). Statistical significance was assessed by ANOVA following Tukey’s multiple comparisons test, ** p < 0.005, * p < 0.05. (B) Comparison of neutralizing activity in sera from individuals who received adenoviral DNA-based vaccines and mRNA-based vaccines. Statistical significance was assessed by unpaired t-test, * p = 0.0016.

Article Snippet: Some of them have high costs, require trained personnel, and can only be carried out in a Biosafety Safety Level 3-equipped laboratory, whereas others, such as the cPass surrogate virus neutralization test (sVNT) (GenScript, Piscataway, NJ, USA) used in this work, are ELISA-based assays and only require optical density readers.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Inhibition, Binding Assay, Comparison, Vaccines

Journal: Frontiers in Immunology

Article Title: Humoral Immune Response Diversity to Different COVID-19 Vaccines: Implications for the “Green Pass” Policy

doi: 10.3389/fimmu.2022.833085

Figure Lengend Snippet: Mean neutralizing activity measured by the cPass™ ELISA-based assay in sera from 70 individuals who received different vaccines.

Article Snippet: Some of them have high costs, require trained personnel, and can only be carried out in a Biosafety Safety Level 3-equipped laboratory, whereas others, such as the cPass surrogate virus neutralization test (sVNT) (GenScript, Piscataway, NJ, USA) used in this work, are ELISA-based assays and only require optical density readers.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Vaccines

Journal: Scientific Reports

Article Title: Evaluation of commercially available fully automated and ELISA-based assays for detecting anti-SARS-CoV-2 neutralizing antibodies

doi: 10.1038/s41598-022-21317-x

Figure Lengend Snippet: Analytical performance of the evaluated sVNT assays using the pre-defined cutoff values and the newly calculated cutoff values determined using Youden's index.

Article Snippet: Further, GenScript cPass sVNT exhibited the best linear correlation with pVNT in samples collected from both infected and vaccinated individuals.

Techniques: Infection

Journal: Scientific Reports

Article Title: A pseudovirus-based platform to measure neutralizing antibodies in Mexico using SARS-CoV-2 as proof-of-concept

doi: 10.1038/s41598-022-22921-7

Figure Lengend Snippet: Anti-SARS-CoV-2 S total IgG underestimates neutralization potential evaluated using the SARS-CoV-2 pseudotyped VP-based assay. ( A ) SARS-CoV-2 S ELISA-based quantification of total IgG in COVID-19 convalescent sera, BNT162b2 vaccinated sera and pre-pandemic sera. ( B ) ID50 of all samples tested with SARS-CoV-2 S pseudoVP assay. Fully colored symbols indicate matched samples also tested in ( A , C , D ). ( C ) Correlation 1:20 sera neutralization potential vs. SARS-CoV-2 S IgG ratio. Purple: COVID-19 convalescent sera, orange: vaccinated sera, p < 0.001 (***) Black: pre-pandemic sera. ( D ) Comparison of COVID-19, BNT162b2 and pre-pandemic sera neutralization with pseudotyped VP-based assay and cPass SARS-CoV-2 ELISA.

Article Snippet: As most published pseudotyped VP-based NAb assays and the surrogate Genscript cPass ELISA assay applied a 30% neutralization cutoff to discriminate positivity, we also applied this arbitrary cutoff for consistency – , .

Techniques: Neutralization, Enzyme-linked Immunosorbent Assay, Comparison

Journal: International Journal of Obesity (2005)

Article Title: The majority of SARS-CoV-2-specific antibodies in COVID-19 patients with obesity are autoimmune and not neutralizing

doi: 10.1038/s41366-021-01016-9

Figure Lengend Snippet: Bars show means ± SE (standard errors) of SARS-CoV-2 Spike-specific IgG antibodies (OD) measured by ELISA. Mean comparisons between groups were performed by one-way ANOVA ( F = 23.76, p = 0.0029). * p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: Neutralizing antibodies were measured by the cPass neutralization ELISA assay, a surrogate plaque reducing neutralization test (sVNT) using the DYNEX Agility® Automated ELISA system.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Obesity (2005)

Article Title: The majority of SARS-CoV-2-specific antibodies in COVID-19 patients with obesity are autoimmune and not neutralizing

doi: 10.1038/s41366-021-01016-9

Figure Lengend Snippet: Neutralizing antibodies were measured by the cPass neutralization ELISA assay, a surrogate plaque reducing neutralization test (sVNT) using the DYNEX Agility® Automated ELISA system. This test allows the identification of neutralizing antibodies with high, medium, or low neutralization potential, corresponding to >5000, 1500–500, and <1500 U/mL, respectively. Bars show means ± SE (standard errors) of Spike-specific neutralizing IgG antibodies (U/mL). Mean comparisons between groups were performed by unpaired Student’s t test. **** p < 0.0001.

Article Snippet: Neutralizing antibodies were measured by the cPass neutralization ELISA assay, a surrogate plaque reducing neutralization test (sVNT) using the DYNEX Agility® Automated ELISA system.

Techniques: Neutralization, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Obesity (2005)

Article Title: The majority of SARS-CoV-2-specific antibodies in COVID-19 patients with obesity are autoimmune and not neutralizing

doi: 10.1038/s41366-021-01016-9

Figure Lengend Snippet: Bars show means ± SE (standard errors) of anti-MDA ( A ) and anti-AD ( B ) IgG antibodies measured by ELISA. Mean comparisons between groups were performed by one-way ANOVA. A ( F = 82.90, p < 0.0001). B ( F = 32.41, p = 0.049). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Neutralizing antibodies were measured by the cPass neutralization ELISA assay, a surrogate plaque reducing neutralization test (sVNT) using the DYNEX Agility® Automated ELISA system.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Obesity (2005)

Article Title: The majority of SARS-CoV-2-specific antibodies in COVID-19 patients with obesity are autoimmune and not neutralizing

doi: 10.1038/s41366-021-01016-9

Figure Lengend Snippet: Bars show means ± SE (standard errors) of CRP serum levels measured by ELISA. Mean comparisons between groups were performed by unpaired Student’s t test. *** p < 0.001.

Article Snippet: Neutralizing antibodies were measured by the cPass neutralization ELISA assay, a surrogate plaque reducing neutralization test (sVNT) using the DYNEX Agility® Automated ELISA system.

Techniques: Enzyme-linked Immunosorbent Assay